Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
特异性
Polyvalent for Shigella antigens
交叉反应 (详细)
Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
纯化方法
This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
免疫原
Mixture of S. boydii, S. flexneri & S. dysenteriae
TITER : Not Determined This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
储存液
Thimerosal (Merthiolate)
注意事项
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.