Borrelia Burgdorferi 抗体 (Peroxidase (POD))
The 兔 多克隆 anti-Borrelia Burgdorferi antibody is suitable to detect Borrelia Burgdorferi in samples from Borrelia burgdorferi. It has been validated for WB, IHC 和 EIA.
产品编号 ABIN7478142
发货至:
中国
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Quick Overview for Borrelia Burgdorferi 抗体 (Peroxidase (POD)) (ABIN7478142)
抗原
Borrelia Burgdorferi
适用
Borrelia burgdorferi
宿主
兔
克隆类型
多克隆
标记
This Borrelia Burgdorferi antibody is conjugated to Peroxidase (POD)
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
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特异性
- Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands
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交叉反应 （详细）
- Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii
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纯化方法
- This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
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免疫原
- Whole cell preparation from B. burgdorferi
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亚型
- IgG
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anti-Borrelia Burgdorferi antibody
适用: Borrelia burgdorferi WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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TITER : >1:1,000 in Western Blot. >1:40,000 in avidin-biotin IFA
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays, Functions well in formalin fixed/paraffin embedded settings -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- Borrelia Burgdorferi
抗原
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