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Recombinant SOX9 抗体 (AA 393-508)

This anti-SOX9 antibody is a 兔 单克隆 antibody detecting SOX9 in IHC. Suitable for 人.
产品编号 ABIN7477587
发货至: 中国

Quick Overview for Recombinant SOX9 抗体 (AA 393-508) (ABIN7477587)

抗原

See all SOX9 抗体
SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This SOX9 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-709R
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    AA 393-508

    免疫原

    Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.

    Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres - if present).

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

    别名

    SOX9

    背景

    Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9,SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P48436

    途径

    EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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