Recombinant SOX9 抗体 (AA 393-508)
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Quick Overview for Recombinant SOX9 抗体 (AA 393-508) (ABIN7470674)
抗原
See all SOX9 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 393-508
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原理
- SOX9 antibody (709R)
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免疫原
- Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.
Product Description: SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres - if present).
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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有效期
- 24 months
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- SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))
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别名
- SOX9
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背景
- Gene Alias: Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9
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UniProt
- P48436
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途径
- EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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