Recombinant SOX9 抗体 (AA 393-508)
Quick Overview for Recombinant SOX9 抗体 (AA 393-508) (ABIN7477587)
抗原
See all SOX9 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 393-508
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免疫原
- Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.
Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres - if present).
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))
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别名
- SOX9
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背景
- Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9,SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P48436
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途径
- EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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