S100A12 抗体
Quick Overview for S100A12 抗体 (ABIN7477583)
抗原
See all S100A12 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Spleen: A significant number of inflammatory cells should show a strong S100A12 immunostaining while the majority of cells remain completely negative.
Negative Control: Colon: All epithelial and muscular cells must not show any S100A12 immunostaining. Few scattered S100A12 positive inflammatory cells can occur (mostly within blood vessels)
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- S100A12 (S100 Calcium Binding Protein A12 (S100A12))
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别名
- S100A12
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背景
- CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6,S100A12 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P80511
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途径
- Toll-Like Receptors Cascades, S100 Proteins
抗原
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