Recombinant MBP 抗体 (AA 150-250)
Quick Overview for Recombinant MBP 抗体 (AA 150-250) (ABIN7477511)
抗原
See all MBP 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 150-250
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免疫原
- Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Brain: A fibrillar staining pattern should be seen.
Negative Control: Colon: MBP staining should be completely absent.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- MBP (Myelin Basic Protein (MBP))
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别名
- Myelin Basic Protein
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背景
- GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP,Myelin Basic Protein antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P02686
抗原
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