GLN1 抗体 (AA 50-250)
Quick Overview for GLN1 抗体 (AA 50-250) (ABIN7477483)
抗原
See all GLN1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 50-250
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原理
- Glutamine Synthetase antibody (750M)
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免疫原
- Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)
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亚型
- IgG2c, kappa
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells.
Product Description/Background: Glutamine Synthetase antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Liver: Periportal hepatocytes must be GS negative in the normal liver.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- GLN1 (Glutamine Synthetase (GLN1))
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别名
- Glutamine Synthetase
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背景
- Gene Alias: cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS
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UniProt
- P15104
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途径
- Positive Regulation of Peptide Hormone Secretion
抗原
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