GLN1 抗体 (AA 50-250)
Quick Overview for GLN1 抗体 (AA 50-250) (ABIN7477483)
抗原
See all GLN1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 50-250
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免疫原
- Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells.
Negative Control: Liver: Periportal hepatocytes must be GS negative in the normal liver.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- GLN1 (Glutamine Synthetase (GLN1))
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别名
- Glutamine Synthetase
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背景
- Cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS,Glutamine Synthetase antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P15104
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途径
- Positive Regulation of Peptide Hormone Secretion
抗原
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