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GATA3 抗体

This anti-GATA3 antibody is a 小鼠 单克隆 antibody detecting GATA3 in IHC. Suitable for 人.
产品编号 ABIN7477479
发货至: 中国

Quick Overview for GATA3 抗体 (ABIN7477479)

抗原

See all GATA3 抗体
GATA3 (GATA Binding Protein 3 (GATA3))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This GATA3 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-450M
  • 免疫原

    Recombinant full-length human GATA-3 protein

    亚型

    IgG
  • 应用备注

    IHC 1:50-1:100

    说明

    Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. , Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.

    Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. , Tonsil: Staining should be absent in B-cells.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    GATA3 (GATA Binding Protein 3 (GATA3))

    别名

    GATA3

    背景

    GATA3, GATA binding protein-3, GATA-binding factor 3, GATA3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-3,GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P23771

    途径

    Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
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