GATA3 抗体
Quick Overview for GATA3 抗体 (ABIN7477479)
抗原
See all GATA3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- Recombinant full-length human GATA-3 protein
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亚型
- IgG
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应用备注
- IHC 1:50-1:100
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说明
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Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. , Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.
Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. , Tonsil: Staining should be absent in B-cells.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- GATA3 (GATA Binding Protein 3 (GATA3))
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别名
- GATA3
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背景
- GATA3, GATA binding protein-3, GATA-binding factor 3, GATA3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-3,GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P23771
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途径
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
抗原
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