F13A1 抗体 (AA 46-181)
Quick Overview for F13A1 抗体 (AA 46-181) (ABIN7477475)
抗原
See all F13A1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 46-181
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免疫原
- Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A fraction of monocytic cells in the mucosa should show a strong cytoplasmic factor XIIIa immunostaining.
Negative Control: Colon: Factor XIIIa immunostaining should be absent in epithelial, endothelial and smooth muscle cells.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))
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别名
- Factor XIII alpha
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背景
- Coagulation factor XIII A chain, Coagulation factor XIII A1 polypeptide, F13A, F13a1, Fibrin stabilizing factor, A subunit, Fibrinoligase, FSF, A subunit, Protein-glutamine gamma-glutamyltransferase A chain, TGase, Transglutaminase. Plasma,Factor XIII alpha antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P00488
抗原
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