Recombinant EpCAM 抗体 (Extracellular Domain)
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Quick Overview for Recombinant EpCAM 抗体 (Extracellular Domain) (ABIN7470615)
抗原
See all EpCAM (EPCAM) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 100-224, Extracellular Domain
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原理
- EpCAM antibody (326R)
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免疫原
- Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Kidney: In kidney, distal tubule cells must show a strong predominantly membranous staining, while at a least moderate predominantly basolateral staining must be seen in the majority of proximal tubules cells and in scattered epithelial cells lining the Bowman capsule.
Product Description: EpCAM antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: EpCAM staining should be absent in lymphocytes or smooth muscle cells of the vessels.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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有效期
- 24 months
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- EpCAM
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背景
- Gene Alias: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-4
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UniProt
- P16422
抗原
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