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ANO1 抗体 (AA 2-101)

This anti-ANO1 antibody is a 小鼠 单克隆 antibody detecting ANO1 in IHC. Suitable for 人.
产品编号 ABIN7477463
发货至: 中国

Quick Overview for ANO1 抗体 (AA 2-101) (ABIN7477463)

抗原

See all ANO1 抗体
ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

适用

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宿主

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小鼠

克隆类型

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  • 1
单克隆

标记

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This ANO1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-201M
  • 抗原表位

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    AA 2-101

    免疫原

    Recombinant human DOG-1 protein fragment (aa 2-101) (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Appendix: An at least moderate, predominantly membranous staining should be seen in Cajal cells in the muscular wall and a weak membranous staining should be seen in columnar epithelial cells in the basal part of the crypts of the appendix.

    Negative Control: Appendix: Staining should be absent in the majority of cells in the muscular wall and in epithelial cells located at the surface.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

    别名

    Dog1

    背景

    Anoctamin 1, Calcium Activated Chloride Channel, Discovered On Gastrointestinal Stromal Tumors Protein 1, TAOS2, ORAOV2, TMEM16A,Dog1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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