Desmoglein 3 抗体 (AA 379-491)
Quick Overview for Desmoglein 3 抗体 (AA 379-491) (ABIN7477461)
抗原
See all Desmoglein 3 (DSG3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 379-491
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免疫原
- Recombinant fragment (around aa 379-491) human DSG3 protein (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:75-1:150
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说明
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Positive Control: Tonsil: A moderate to strong desmoglein 3 immunostaining should be seen in a fraction of squamous epithelial cells.
Negative Control: Tonsil: Desmoglein 3 immunostaining should be absent in all non-squamous epithelial cell types.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Desmoglein 3 (DSG3)
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别名
- Desmoglein 3
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背景
- 130 kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3),Desmoglein 3 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P32926
抗原
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