Recombinant CD3 抗体 (AA 20-40)
Quick Overview for Recombinant CD3 抗体 (AA 20-40) (ABIN7477391)
抗原
See all CD3 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 20-40
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免疫原
- A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells).
Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD3
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别名
- CD3
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背景
- CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P07766
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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