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Recombinant CD3 抗体 (AA 20-40)

This anti-CD3 antibody is a 兔 单克隆 antibody detecting CD3 in IHC. Suitable for 人.
产品编号 ABIN7477391
发货至: 中国

Quick Overview for Recombinant CD3 抗体 (AA 20-40) (ABIN7477391)

抗原

See all CD3 抗体
CD3

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This CD3 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-003R
  • 抗原表位

    • 3
    • 2
    • 1
    • 1
    • 1
    AA 20-40

    免疫原

    A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells).

    Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    CD3

    别名

    CD3

    背景

    CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P07766

    途径

    TCR Signaling, Ubiquitin Proteasome Pathway
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