CALB1 抗体 (AA 7-96)
Quick Overview for CALB1 抗体 (AA 7-96) (ABIN7477383)
抗原
See all CALB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 7-96
-
免疫原
- Recombinant fragment (around aa 7-96) of human CALB1 protein (exact sequence is proprietary)
-
亚型
- IgG
-
-
-
-
应用备注
- IHC 1:50-1:100
-
说明
-
Positive Control: Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining.
Negative Control: Colon: Calbindin 1 immunostaining should be completely absent in all cells.
-
实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- CALB1 (Calbindin (CALB1))
-
别名
- Calbindin 1
-
背景
- Avian-type, CAB27, CALB 1, CALB, CALB1, CALB1_HUMAN, Calbindin 1 28 kDa, Calbindin, Calbindin D28, D 28K, D-28K, D28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL H protein, Vitamin D dependent calcium binding protein, Vitamin D dependent calcium binding protein avian type, Vitamin D-dependent calcium-binding protein,Calbindin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P05937
抗原
-