Iba1 抗体 (AA 1-146)
Quick Overview for Iba1 抗体 (AA 1-146) (ABIN7477371)
抗原
See all Iba1 (IBA1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-146
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免疫原
- Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages.
Negative Control: Colon: AIF1 immunostaining should be absent in all non-inflammatory cells.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
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别名
- AIF1
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背景
- Allograft inflammatory factor 1 (AIF1), balloon angioplasty responsive transcription (BART1), IBA1, Interferon responsive transcript 1, Ionized calcium-binding adapter molecule 1, IRT1, Microglia response factor (MRF1),AIF1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P55008
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途径
- Smooth Muscle Cell Migration
抗原
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