RbAp48 + RbAp46 (AA 1-425) 抗体
This 小鼠 单克隆 antibody specifically detects RbAp48 + RbAp46 in EMSA, ICC, IF, IHC (p), IP 和 WB. It exhibits reactivity toward 人.
产品编号 ABIN7469571
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for RbAp48 + RbAp46 (AA 1-425) 抗体 (ABIN7469571)
抗原
RbAp48 + RbAp46
适用
人
宿主
小鼠
克隆类型
单克隆
应用范围
Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
克隆位点
15G12
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抗原表位
- AA 1-425
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交叉反应
- 人, 小鼠
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纯化方法
- Protein G purified
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免疫原
- The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
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亚型
- IgG2a
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 0.2 - 1 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: K562 , THP-1 , HL-60
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1XPBS ( pH 7), 20 % Glycerol, No Preservative
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- RbAp48 + RbAp46
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背景
- RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.
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分子量
- 46 and 48 kDa
抗原
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