Protein phosphatase 1 (Center) 抗体
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北京 101111
Quick Overview for Protein phosphatase 1 (Center) 抗体 (ABIN7468810)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Center
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交叉反应
- 人
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human MYPT1. The exact sequence is proprietary.
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亚型
- IgG
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anti-Protein phosphatase 1 (AA 433-636), (Regulatory Subunit 15A) antibody
适用: 小鼠 WB, IHC, IF/ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein phosphatase 1 (AA 335-578), (Regulatory Subunit 15A) antibody适用: 大鼠 WB, IHC, IF/ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Protein phosphatase 1
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背景
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Synonyms: protein phosphatase 1 regulatory subunit 12A , M130 , MBS , MYPT1
Background: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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分子量
- 115 kDa
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基因ID
- 4659
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UniProt
- O14974
抗原
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