Protein A 抗体 (HRP)
Quick Overview for Protein A 抗体 (HRP) (ABIN7468794)
抗原
See all Protein A 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Chickens were immunized with purified recombinant protein A. After multiple immunizations in Freund's adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with horseradish peroxidase (HRP).
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亚型
- IgY
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应用备注
- For ELISA: Use at a dilution of 1:5,000-1:20,000. For WB: Use at a dilution of 1:1,000-1:20,000. Optimal dilutions/concentrations should be determined by the researcher.
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说明
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Positive Control: Protein A
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.43 mg/mL
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缓冲液
- 0.05M Tris ( pH 7.4), 0.15M NaCl, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Protein A
抗原
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