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EIF2S1 抗体 (pSer52)

This 兔 多克隆 antibody specifically detects EIF2S1 in WB. It exhibits reactivity toward 人.
产品编号 ABIN7468320
发货至: 中国
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Quick Overview for EIF2S1 抗体 (pSer52) (ABIN7468320)

抗原

See all EIF2S1 抗体
EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

适用

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宿主

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克隆类型

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多克隆

标记

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This EIF2S1 antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

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    pSer52

    交叉反应

    人, 大鼠

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Carrier-protein conjugated synthetic peptide surrounding phospho Ser52 of human eIF2 alpha. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: PC-12 (300 μM Thapsigargin treatment for 8hr) , 293T (25 μg/ml anisomycin treatment for 16hr) Validation: Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.77 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

    别名

    eukaryotic translation initiation factor 2 subunit alpha

    背景

    Synonyms: eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

    Background: The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

    分子量

    36 kDa

    基因ID

    1965

    UniProt

    P05198

    途径

    Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C
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