ERO1L 抗体
Quick Overview for ERO1L 抗体 (ABIN7468316)
抗原
See all ERO1L 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应
- 小鸡, 人, 小鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human ERO1L. The exact sequence is proprietary.
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亚型
- IgG
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anti-ERO1-Like Protein alpha (ERO1L) (AA 90-178) antibody
ERO1L 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4G3 unconjugated
anti-ERO1-Like Protein alpha (ERO1L) (AA 90-178) antibodyERO1L 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-ERO1-Like Protein alpha (ERO1L) (AA 24-260) antibodyERO1L 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ERO1-Like Protein alpha (ERO1L) (AA 105-118) antibodyVerified ERO1L 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-ERO1-Like Protein alpha (ERO1L) (AA 321-420) antibodyERO1L 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-ERO1-Like Protein alpha (ERO1L) (AA 1-468) antibodyERO1L 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-ERO1-Like Protein alpha (ERO1L) (AA 1-468) antibodyERO1L 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-ERO1-Like Protein alpha (ERO1L) (AA 350-379), (C-Term) antibodyERO1L 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33290 unconjugated
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: mouse stomach , HCT116 , MCF-7
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.95 mg/mL
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缓冲液
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- ERO1L (ERO1-Like Protein alpha (ERO1L))
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别名
- endoplasmic reticulum oxidoreductase 1 alpha
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背景
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Synonyms: endoplasmic reticulum oxidoreductase 1 alpha , ERO1-L , ERO1-L-alpha , ERO1-alpha , ERO1L , ERO1LA , Ero1alpha
Background: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
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分子量
- 54 kDa
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基因ID
- 30001
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UniProt
- Q96HE7
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途径
- Peptide Hormone Metabolism, ER-Nucleus Signaling, Brown Fat Cell Differentiation
抗原
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