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DBT 抗体

This 兔 多克隆 anti-DBT antibody specifically detects DBT in WB, IF, ICC 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7467383
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for DBT 抗体 (ABIN7467383)

抗原

See all DBT 抗体
DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

适用

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宿主

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克隆类型

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多克隆

标记

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This DBT antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human DBT. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: rat liver , H1299 , HepG2 , mouse liver

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.3 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

    别名

    dihydrolipoamide branched chain transacylase E2

    背景

    Dihydrolipoamide branched chain transacylase E2 , BCATE2 , BCKAD-E2 , BCKADE2 , BCOADC-E2 , E2 , E2B,The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

    分子量

    53 kDa

    基因ID

    1629

    UniProt

    P11182
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