ETF1 抗体 (N-Term)
Quick Overview for ETF1 抗体 (N-Term) (ABIN7467264)
抗原
See all ETF1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
交叉反应
- 人, 小鼠, 斑马鱼
-
纯化方法
- Purified by antigen-affinity chromatography.
-
免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human eRF1. The exact sequence is proprietary.
-
亚型
- IgG
-
-
anti-Eukaryotic Translation Termination Factor 1 (ETF1) (AA 338-437) antibody
ETF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H4 unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) antibodyETF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) antibodyETF1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) (AA 278-437) antibodyETF1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) (AA 35-84) antibodyETF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 斑马鱼, 豚鼠, 马, 兔, 非洲爪蟾, 猴, Pig, Hamster, Drosophila melanogaster, 小鸡, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) (N-Term) antibodyETF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 斑马鱼, 豚鼠, 马, 兔, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
-
说明
-
Positive Control: Raji
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.8 mg/mL
-
缓冲液
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
-
储存液
- Thimerosal (Merthiolate)
-
注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
-
- ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
-
别名
- eukaryotic translation termination factor 1
-
背景
- Eukaryotic translation termination factor 1 , D5S1995 , ERF , ERF1 , RF1 , SUP45L1 , TB3-1,Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]
-
分子量
- 49 kDa
-
基因ID
- 2107
-
UniProt
- P62495
抗原
-
