BCKDHA 抗体 (C-Term)
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Quick Overview for BCKDHA 抗体 (C-Term) (ABIN7466051)
抗原
See all BCKDHA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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交叉反应
- 人
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA. The exact sequence is proprietary.
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亚型
- IgG
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anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (pSer292) antibody
BCKDHA 适用: 人, 小鼠 WB 宿主: 兔 Monoclonal BLR060F unconjugated Recombinant Antibody
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 30-445) antibodyBCKDHA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (pSer292) antibodyBCKDHA 适用: 人, 小鼠 WB 宿主: 兔 Monoclonal BLR060F unconjugated Recombinant Antibody
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 268-407) antibodyBCKDHA 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 347-445) antibodyBCKDHA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (pSer292) antibodyBCKDHA 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 362-390), (C-Term) antibodyBCKDHA 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20924 unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 1-445) antibodyBCKDHA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (N-Term) antibodyBCKDHA 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 276-445) antibodyBCKDHA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: HepG2
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
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别名
- branched chain keto acid dehydrogenase E1, alpha polypeptide
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背景
- Branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A,The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD, EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2, MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3, MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM]
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分子量
- 50 kDa
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基因ID
- 593
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UniProt
- P12694
抗原
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