MANEA 抗体
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- 抗原 See all MANEA 抗体
- MANEA (Mannosidase, Endo-alpha (MANEA))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MANEA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人, 小鼠
- 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human MANEA. The exact sequence is proprietary.
- 亚型
- IgG
- Top Product
- Discover our top product MANEA Primary Antibody
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anti-Mannosidase, Endo-alpha (MANEA) antibody
MANEA 适用: 人 IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Mannosidase, Endo-alpha (MANEA) (AA 218-267) antibodyMANEA 适用: 人, 小鼠, 大鼠, 犬, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Mannosidase, Endo-alpha (MANEA) (AA 30-100) antibodyMANEA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Mannosidase, Endo-alpha (MANEA) (Middle Region) antibodyMANEA 适用: 人, 小鼠, 大鼠, 犬, 马, 斑马鱼, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: A431 , HeLa , HepG2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- MANEA (Mannosidase, Endo-alpha (MANEA))
- 别名
- mannosidase endo-alpha (MANEA 产品)
- 别名
- ENDO antibody, hEndo antibody, Enman antibody, 4932703L02Rik antibody, fi29h09 antibody, zgc:92825 antibody, wu:fi29h09 antibody, mannosidase endo-alpha antibody, mannosidase, endo-alpha antibody, MANEA antibody, Manea antibody, manea antibody
- 背景
- Mannosidase endo-alpha , ENDO , hEndo,N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM]
- 分子量
- 54 kDa
- 基因ID
- 79694
- UniProt
- Q5SRI9
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