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MANEA 抗体

This anti-MANEA antibody is a 兔 多克隆 antibody detecting MANEA in WB 和 IHC (p). Suitable for 人.
产品编号 ABIN7465447
发货至: 中国

Quick Overview for MANEA 抗体 (ABIN7465447)

抗原

See all MANEA 抗体
MANEA (Mannosidase, Endo-alpha (MANEA))

适用

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宿主

  • 5

克隆类型

  • 5
多克隆

标记

  • 5
This MANEA antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 交叉反应

    人, 小鼠

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human MANEA. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: A431 , HeLa , HepG2

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    MANEA (Mannosidase, Endo-alpha (MANEA))

    别名

    mannosidase endo-alpha

    背景

    Mannosidase endo-alpha , ENDO , hEndo,N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM]

    分子量

    54 kDa

    基因ID

    79694

    UniProt

    Q5SRI9
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