PC 抗体 (C-Term)
Quick Overview for PC 抗体 (C-Term) (ABIN7465195)
抗原
See all PC 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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交叉反应
- 人, 大鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:857. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: 293T , A431 , HeLa , HepG2
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.69 mg/mL
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缓冲液
- 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- PC (Pyruvate Carboxylase (PC))
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别名
- pyruvate carboxylase
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背景
- Pyruvate carboxylase , PCB,This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]
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分子量
- 130 kDa
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基因ID
- 5091
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UniProt
- P11498
抗原
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