Cyclin B1 抗体
Quick Overview for Cyclin B1 抗体 (ABIN7464490)
抗原
See all Cyclin B1 (CCNB1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
交叉反应
- 人
-
纯化方法
- Affinity purified by Protein G.
-
免疫原
- Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.
-
亚型
- IgG1
-
-
-
-
应用备注
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
-
说明
-
Positive Control: HeLa (2mM thymidine treatment and recovery for 3.5hr) , HeLa (2mM thymidine treatment and recovery for 12hr) , U2OS (100ng/ml Nocodazole treatment for 24hr)
Validation: Orthogonal
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS, No Preservative
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
-
- Cyclin B1 (CCNB1)
-
别名
- cyclin B1
-
背景
- Cyclin B1 , CCNB,The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]
-
分子量
- 48 kDa
-
基因ID
- 891
-
UniProt
- P14635
-
途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
抗原
-