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Cyclin B1 抗体

This 小鼠 单克隆 anti-Cyclin B1 antibody (Clone GT238) specifically detects Cyclin B1 in WB 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7464490
发货至: 中国
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Quick Overview for Cyclin B1 抗体 (ABIN7464490)

抗原

See all Cyclin B1 (CCNB1) 抗体
Cyclin B1 (CCNB1)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cyclin B1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

GT238
  • 交叉反应

    纯化方法

    Affinity purified by Protein G.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

    亚型

    IgG1
  • 应用备注

    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: HeLa (2mM thymidine treatment and recovery for 3.5hr) , HeLa (2mM thymidine treatment and recovery for 12hr) , U2OS (100ng/ml Nocodazole treatment for 24hr)

    Validation: Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, No Preservative

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    Cyclin B1 (CCNB1)

    别名

    cyclin B1

    背景

    Cyclin B1 , CCNB,The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

    分子量

    48 kDa

    基因ID

    891

    UniProt

    P14635

    途径

    Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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