Cyclin B1 抗体
Quick Overview for Cyclin B1 抗体 (ABIN7464490)
抗原
See all Cyclin B1 (CCNB1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应
 - 人
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纯化方法
 - Affinity purified by Protein G.
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免疫原
 - Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.
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亚型
 - IgG1
 
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应用备注
 - WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: HeLa (2mM thymidine treatment and recovery for 3.5hr) , HeLa (2mM thymidine treatment and recovery for 12hr) , U2OS (100ng/ml Nocodazole treatment for 24hr)
Validation: Orthogonal
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 1 mg/mL
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缓冲液
 - PBS, No Preservative
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储存液
 - Without preservative
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储存条件
 - 4 °C,-20 °C
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储存方法
 - Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
 
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- Cyclin B1 (CCNB1)
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别名
 - cyclin B1
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背景
 - Cyclin B1 , CCNB,The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]
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分子量
 - 48 kDa
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基因ID
 - 891
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UniProt
 - P14635
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途径
 - Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
 
抗原
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