CDK1 抗体 (pTyr15)
Quick Overview for CDK1 抗体 (pTyr15) (ABIN7464489)
抗原
See all CDK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr15
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交叉反应
- Drosophila melanogaster, 人
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide surrounding phospho Tyr15 of human CDC2. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: HeLa (2mM double thymidine treatment and recovery for 3.5hr) , HeLa (100ng/ml Nocodazole treatment for 24hr)
Validation: Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.84 mg/mL
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缓冲液
- 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
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别名
- cyclin dependent kinase 1
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背景
- Cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2,The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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分子量
- 34 kDa
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基因ID
- 983
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UniProt
- P06493
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
抗原
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