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GCLM 抗体 (N-Term)

The 兔 多克隆 anti-GCLM antibody is suitable to detect GCLM in samples from 人. It has been validated for WB, IF, ICC 和 IHC (p).
产品编号 ABIN7464409
发货至: 中国
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Quick Overview for GCLM 抗体 (N-Term) (ABIN7464409)

抗原

See all GCLM 抗体
GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

适用

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宿主

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克隆类型

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多克隆

标记

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This GCLM antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

质量等级

KO Validated
  • 抗原表位

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    N-Term

    交叉反应

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GCLM. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: H1299 , HeLa

    Validation: KO/KD

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

    别名

    glutamate-cysteine ligase modifier subunit

    背景

    Glutamate-cysteine ligase modifier subunit , GLCLR,Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

    分子量

    31 kDa

    基因ID

    2730

    UniProt

    P48507
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