AFT1 抗体 (pSer63)
Quick Overview for AFT1 抗体 (pSer63) (ABIN7464253)
抗原
See all AFT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer63
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特异性
- This antibody is raised against human ATF1 phosphorylated at Ser63, and it also may detect the phosphorylated CREB protein due to sequence homology.
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交叉反应
- 人, 小鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide surrounding phospho Ser63 of human ATF1. The exact sequence is proprietary.
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亚型
- IgG
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anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibody
AFT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 适用: 人 WB, ELISA, IHC (p), RNAi 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 3-245) antibodyAFT1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (Internal Region) antibodyAFT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (C-Term) antibodyAFT1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 3-213) antibodyAFT1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-210) antibodyAFT1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (pSer63) antibodyAFT1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-272) antibodyAFT1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3E7 unconjugated
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应用备注
- WB: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: 293T (25 μg/ml anisomycin treatment for 30 min) , A431(200 ng/ml EGF treatment for 30 min) , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Validation: Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.94 mg/mL
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缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- AFT1 (Activating Transcription Factor 1 (AFT1))
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别名
- activating transcription factor 1
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背景
- Activating transcription factor 1 , EWS-ATF1 , FUS/ATF-1 , TREB36,This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq]
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分子量
- 29 kDa
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基因ID
- 466
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UniProt
- P18846
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途径
- Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades
抗原
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