RDBP 抗体 (AA 330-380)
Quick Overview for RDBP 抗体 (AA 330-380) (ABIN7454422)
抗原
See all RDBP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 330-380
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原理
- Rabbit anti-NELFE Antibody, Affinity Purified
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预测反应
- Bovine
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纯化方法
- Affinity Purified
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免疫原
- between AA 330 and 380
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亚型
- IgG
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应用备注
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IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 5 μg/mg lysate
WB: Not recommended. A301-914A has not performed satisfactorily when used for WB of NELFE in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NELFE.
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- RDBP (RD RNA Binding Protein (RDBP))
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别名
- NELFE
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背景
- Background: NELFE (negative elongation factor E) is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. NELF-E is an RNA-binding protein. The RNA binding activity of NELF-E is critical to the function of NELF.
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基因ID
- 7936
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NCBI登录号
- NP_002895
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UniProt
- P18615
抗原
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