MED1 抗体 (C-Term)
Quick Overview for MED1 抗体 (C-Term) (ABIN7453585)
抗原
See all MED1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Rabbit anti-MED1 Antibody, Affinity Purified
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预测反应
- Orangutan
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纯化方法
- Affinity Purified
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免疫原
- between AA 1525 and C-term
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亚型
- IgG
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应用备注
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IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 10 μg/mg lysate
WB: 1:2,000 - 1:10,000
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- MED1 (Mediator Complex Subunit 1 (MED1))
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别名
- MED1
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背景
- Background: MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) Q15648].
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基因ID
- 5469
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NCBI登录号
- NP_004765
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UniProt
- Q15648
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途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
抗原
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