MED12 抗体 (C-Term)
Quick Overview for MED12 抗体 (C-Term) (ABIN7453548)
抗原
See all MED12 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Rabbit anti-MED12 Antibody, Affinity Purified
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预测反应
- Chimpanzee
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纯化方法
- Affinity Purified
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免疫原
- between AA 2127 and C-term
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亚型
- IgG
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应用备注
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IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 10 μg/mg lysate
WB: 1:2,000 - 1:10,000
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- MED12 (Mediator Complex Subunit 12 (MED12))
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别名
- MED12
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背景
- Background: MED12 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. In this context, MED12 interaction with beta-catenin has been shown to be required for Wnt-signaling dependent transcription.
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基因ID
- 9968
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NCBI登录号
- NP_005111
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UniProt
- Q93074
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation
抗原
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