UBE2L3 抗体 (C-Term)
Quick Overview for UBE2L3 抗体 (C-Term) (ABIN7453475)
抗原
See all UBE2L3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Rabbit anti-UBCH7 Antibody, Affinity Purified
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预测反应
- Bovine,Orangutan
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纯化方法
- Affinity Purified
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免疫原
- between AA 100 and C-term
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亚型
- IgG
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应用备注
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IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 5 μg/mg lysate (efficiency in IP is low)
WB: 1:2,000 - 1:10,000
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
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别名
- UBCH7
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背景
- Background: Ubiquitin conjugating enzyme H7 (UBCH7) is an E2 enzyme of the three step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ubiquitination provides an important post-translational means to regulate protein abundance and activity. UBCH7 has been shown to be involved in the ubiquitination of p53.
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基因ID
- 7332
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NCBI登录号
- NP_003338
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UniProt
- P68036
抗原
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