RNA Polymerase II 抗体 (AA 754-804)
This anti-RNA Polymerase II antibody is a 兔 多克隆 antibody detecting RNA Polymerase II in IP 和 ChIP. Suitable for 人.
Quick Overview for RNA Polymerase II 抗体 (AA 754-804) (ABIN7453315)
抗原
RNA Polymerase II
适用
人
宿主
兔
克隆类型
多克隆
标记
This RNA Polymerase II antibody is un-conjugated
应用范围
Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
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抗原表位
- AA 754-804
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原理
- Rabbit anti-RNA Polymerase II Antibody, Affinity Purified
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预测反应
- Mouse
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纯化方法
- Affinity Purified
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免疫原
- Between AA 754 and 804
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亚型
- IgG
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应用备注
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IP: 2 - 10 μg/mg lysate
WB: Not recommended. ABIN7453315 has not performed satisfactorily when used for WB of RNA Polymerase II in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNA Polymerase II.
ChIP-PCR: 8 μg per IP
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- RNA Polymerase II
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背景
- Background: RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].
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基因ID
- 5430
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NCBI登录号
- NP_000928
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UniProt
- P24928
抗原
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