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gamma H2AX 抗体 (Ser140)

GAMMA-H2AX 适用: 人, 小鼠 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN7449557
发货至: 中国
  • 抗原 See all gamma H2AX (GAMMA-H2AX) products
    gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))
    抗原表位
    • 4
    • 2
    • 1
    Ser140
    适用
    • 5
    • 4
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 7
    克隆类型
    • 4
    • 3
    多克隆
    标记
    • 7
    This gamma H2AX antibody is un-conjugated
    应用范围
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)
    原理
    Rabbit anti-gamma-H2AX Antibody, Affinity Purified
    纯化方法
    Affinity Purified
    免疫原
    surrounding serine 140
    亚型
    IgG
  • 应用备注

    ICC-IF: 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

    IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    WB: 1:10,000 - 1:25,000

    限制
    仅限研究用
  • 浓度
    1000 μg/mL
    缓冲液
    Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))
    别名
    gamma-H2AX (GAMMA-H2AX 产品)
    背景
    Background: H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.
    基因ID
    3014
    NCBI登录号
    NP_002096
    UniProt
    P16104
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