ZEBOV GP 抗体
This anti-ZEBOV GP antibody is a 小鼠 单克隆 antibody detecting ZEBOV GP in ELISA. Suitable for Zaire ebolavirus.
Quick Overview for ZEBOV GP 抗体 (ABIN7383905)
抗原
ZEBOV GP
(Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
适用
Zaire ebolavirus
宿主
小鼠
克隆类型
单克隆
标记
This ZEBOV GP antibody is un-conjugated
应用范围
ELISA
克隆位点
2
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特异性
- Anti-Ebola virus EBOV(subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein/GP Monoclonal Antibody
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纯化方法
- Protein A Affinity
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免疫原
- Recombinant EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His Tag), ABIN7198910
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亚型
- IgG1
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应用备注
- ELISA 1:1000-1:2000
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 0.2 μm filtered solution in PBS
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
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别名
- ZEBOV Glycoprotein/GP
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背景
- Glycoprotein,GP,The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
抗原
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