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tdTomato 抗体

The 山羊 多克隆 anti-tdTomato antibody (ABIN7273105) specifically detects tdTomato in IF, IHC (fro), WB, IEM 和 IHC (p). The antibody is reactive with Discosoma 和 Tomato samples.
产品编号 ABIN7273105
发货至: 中国
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Quick Overview for tdTomato 抗体 (ABIN7273105)

抗原

tdTomato (tdTomato Fluorescent Protein (tdTomato))

适用

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Discosoma, Tomato

宿主

  • 8
山羊

克隆类型

  • 8
多克隆

标记

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This tdTomato antibody is un-conjugated

应用范围

Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 原理

    anti-tdTomato

    序列

    MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

    特异性

    In 293HEK cells transfected with cds plasmid detects a band of 54 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

    交叉反应 (详细)

    Reacts against tdTomato, mCherry, RFP

    产品特性

    Red Fluorescent Protein

    纯化方法

    Epitope affinity purified

    免疫原

    Purified recombinant peptide produced in E. coli.

    亚型

    IgG
  • 应用备注

    WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

    限制

    仅限研究用
  • 浓度

    2 mg/mL

    缓冲液

    preservatives

    注意事项

    The antibody solution should be gently mixed before use.

    储存条件

    4 °C,-20 °C

    储存方法

    For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
  • 抗原

    tdTomato (tdTomato Fluorescent Protein (tdTomato))

    别名

    tdTomato

    背景

    Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It is a ~54 kDa protein that is optimally excited at a 554 nm and has a maximum of emission at 581 nm. It´s brightness and emission wavelength, makes it ideal for live animal research.

    red fluorescent protein antibody.

    分子量

    54 kDa
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