mRuby 抗体
This anti- antibody is a 山羊 多克隆 antibody detecting in IF, IEM, IHC (fro), IHC (p) 和 WB. Suitable for Discosoma.
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Quick Overview for mRuby 抗体 (ABIN7273093)
抗原
mRuby
适用
Discosoma
宿主
山羊
克隆类型
多克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Immunoelectron Microscopy (IEM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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交叉反应 （详细）
- Reacts against Red Fluorescent Protein
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纯化方法
- Epitope affinity purified
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免疫原
- Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
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亚型
- IgG
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anti-mRuby antibody (Atto 488)
适用: Entacmaea quadricolor IF, ICC, FACS 宿主: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
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应用备注
- WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
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说明
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In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
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限制
- 仅限研究用
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浓度
- 3 mg/mL
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缓冲液
- PBS, 20 % glycerol and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.
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- mRuby
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背景
- MRuby (Red fluorescent protein) is a basic (constitutively fluorescent) red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. mRuby is a ~26 kDa protein that is optimally excited at a 558 nm and has a maximum of emission at 605 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
抗原
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