mRuby 抗体
The 山羊 多克隆 anti-mRuby antibody (ABIN7273093) specifically detects mRuby in IF, IEM, IHC (fro), IHC (p) 和 WB.
The antibody is reactive with Entacmaea quadricolor samples.
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产品编号 ABIN7273093
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for mRuby 抗体 (ABIN7273093)
抗原
mRuby
适用
Entacmaea quadricolor
宿主
山羊
克隆类型
多克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Immunoelectron Microscopy (IEM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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交叉反应 （详细）
- Reacts against Red Fluorescent Protein
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纯化方法
- Epitope affinity purified
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免疫原
- Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
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亚型
- IgG
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anti-mRuby antibody (Atto 488)
适用: Entacmaea quadricolor IF, ICC, FACS 宿主: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
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应用备注
- WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
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说明
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In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
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限制
- 仅限研究用
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浓度
- 3 mg/mL
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缓冲液
- PBS, 20 % glycerol and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.
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- mRuby
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背景
- MRuby (Red fluorescent protein) is a basic (constitutively fluorescent) red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. mRuby is a ~26 kDa protein that is optimally excited at a 558 nm and has a maximum of emission at 605 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
抗原
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