mApple Fluorescent Protein 抗体
The 山羊 多克隆 anti-mApple Fluorescent Protein antibody is suitable to detect mApple Fluorescent Protein in samples from Discosoma. It has been validated for IEM, IF, IHC (fro), IHC (p) 和 WB.
产品编号 ABIN7273083
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for mApple Fluorescent Protein 抗体 (ABIN7273083)
抗原
mApple Fluorescent Protein
适用
Discosoma
宿主
山羊
克隆类型
多克隆
应用范围
Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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原理
- anti-mApple
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特异性
- In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
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交叉反应 （详细）
- Reacts against Red Fluorescent Protein
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产品特性
- Red Fluorescent Protein
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纯化方法
- Epitope affinity purified
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免疫原
- Purified recombinant fluorescent protein produced in E. coli.
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亚型
- IgG
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应用备注
- WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
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限制
- 仅限研究用
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浓度
- 3 mg/mL
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缓冲液
- PBS, 20 % glycerol and 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody solution should be gently mixed before use.
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储存条件
- 4 °C,-20 °C
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储存方法
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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- mApple Fluorescent Protein
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背景
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Goat polyclonal antibody to mApple (Apple fluorescent protein). mApple is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mApple is a ~27 kDa protein that is optimally excited at a 568 nm and has a maximum of emission at 592 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
red fluorescent protein, Apple antibody.
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分子量
- 27 kDa
抗原
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