Recombinant ALFA-Tag 抗体 (Alexa Fluor 647)
This anti- antibody is a Alpaca 单克隆 antibody detecting in WB, FACS, ICC, IF 和 IHC. Suitable for .
Quick Overview for Recombinant ALFA-Tag 抗体 (Alexa Fluor 647) (ABIN7272960)
抗原
ALFA-Tag
抗体类型
Recombinant Antibody
片段
single-domain Antibody (sdAb)
宿主
Alpaca
克隆类型
单克隆
标记
Alexa Fluor 647
应用范围
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
克隆位点
1G5
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Expression System
- HEK-293 Cells
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序列
- SRLEEELRRRLTE
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特异性
- ALFA-tag
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纯化方法
- Produced in: HEK
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免疫原
- Single-domain antibody (sdAb) that recognizes a small rationally-designed epitope tag: the ALFA tag.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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应用备注
- 1:500 (corresponding to 5 nM final concentration). ALFA tag and the companion FluoTags-X2 anti-ALFA and ALFA-Catcher are the perfect choices for fluorescent-based experiments that want to be combined with biochemical purifications, mass-spectrometry, or any other high-end application.
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说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.
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缓冲液
- lyophilized from PBS pH7.4 with 2% BSA (US-Origin)
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- ALFA-Tag
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物质类
- Tag
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背景
- Owing to the small size of our FluoTags and the short ALFA-tag (15 amino acids; PSRLEEELRRRLTEP), the distance between the target epitope and each fluorophore is below 3 nm. Proteins of interest can be tagged with our ALFA-tag at the N-termini, C-termini, or in-between domains and they can also be immunoprecipitated or natively purified with complexes or partner binders using our series of ALFA-Catcher resins with physiological elution capability.
抗原
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