Recombinant mEos 抗体 (DBCO)
适用: Lobophyllia hemprichii
ICC, IF
宿主: Alpaca
Monoclonal
1E8
DBCO
single-domain Antibody (sdAb)
Recombinant Antibody
-
- 抗原
- mEos
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
- 适用
- Lobophyllia hemprichii
-
宿主
-
Alpaca
- Expression System
- E.coli
-
克隆类型
- 单克隆
-
标记
- DBCO
-
应用范围
- Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Recognizes mEOS and mEOS derivatives
- 纯化方法
- Produced in: E.coli
- 免疫原
- sdAb anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.
- 克隆位点
- 1E8
-
anti-mEos antibody (Atto 488)
适用: Lobophyllia hemprichii ICC, IF, FACS 宿主: Alpaca Monoclonal 1E8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
-
- 应用备注
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- 缓冲液
- sdAbs are lyophilized from PBS pH 7.4.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
-
- 抗原
- mEos
- 背景
- In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several improved versions were discovered expanding the repertoir of photoswitchable fluorescent probes.
-
You are here:
