Recombinant GFAP 抗体 (Atto 488)
Quick Overview for Recombinant GFAP 抗体 (Atto 488) (ABIN7272868)
抗原
See all GFAP 抗体抗体类型
片段
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Expression System
- E.coli
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特异性
- Recognizes all isotypes from mouse and rat GFAP. Other species not tested
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纯化方法
- Produced in: E.coli
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免疫原
- A fluorescently conjugated single-domain antibody that recognizes mouse and rat GFAP. The single-domain antibody carries two fluorophores and is equipped with specially designed linkers to work in expansion microscopy or enhance post-staining fixation for applications like dSTORM and DNA-PAINT.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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anti-Glial Fibrillary Acidic Protein (GFAP) (AA 51-150) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, ICC, ELISA, FACS, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyGFAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 93-432) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 253-354) antibodyGFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 1-430) antibodyGFAP 适用: 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 292-432) antibodyGFAP 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 小鼠 Monoclonal 1C91F1 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyVerified GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (pSer38) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) antibodyGFAP 适用: 大鼠, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 108-211) antibodyGFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- IF: 1:500 (corresponding to 5 nM final concentration)
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说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.
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缓冲液
- lyophilized from PBS pH7.4 with 2% BSA (US-Origin)
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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别名
- GFAP
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背景
- Glial fibrillary acidic protein (GFAP) belongs to type III intermediate filaments (like vimentin) and is expressed in various cell types throughout the human body. However, in the central nervous system, it is almost exclusively present in astrocytes, resulting in an important marker to differentiate neurons from astrocytes. GFAP is used primarily as an excellent astrocyte cellular marker.
抗原
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