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Recombinant SARS-CoV-2 Nucleocapsid 抗体 (Atto 488)

The Atto 488-conjugated Alpaca 单克隆 anti-SARS-CoV-2 Nucleocapsid antibody (Clone 2A7) (ABIN7272862) specifically detects SARS-CoV-2 Nucleocapsid in IF 和 ICC. The antibody is reactive with SARS Coronavirus-2 (SARS-CoV-2) samples.
产品编号 ABIN7272862
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid 抗体 (Atto 488) (ABIN7272862)

抗原

See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

抗体类型

Recombinant Antibody

片段

single-domain Antibody (sdAb)

适用

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SARS Coronavirus-2 (SARS-CoV-2)

宿主

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Alpaca

克隆类型

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单克隆

标记

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This SARS-CoV-2 Nucleocapsid antibody is conjugated to Atto 488

应用范围

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Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

2A7
  • Expression System

    E.coli

    特异性

    Nucleocapsid from SARS-CoV-2 and SARS-CoV-1

    纯化方法

    Produced in: E.coli

    Labeling Ratio

    a single fluorophore is coupled to exactly one sdAb
  • 应用备注

    IF: 1:500 (Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g., paraffin-embedded tissue.)

    说明

    Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

    缓冲液

    lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

    储存条件

    4 °C/-20 °C/-80 °C

    储存方法

    Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
  • 抗原

    SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

    别名

    Nucleocapsid from SARS-CoV-2

    背景

    The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.
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