Recombinant SARS-CoV-2 Nucleocapsid 抗体 (Alexa Fluor 647)
Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid 抗体 (Alexa Fluor 647) (ABIN7272861)
抗原
See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体抗体类型
片段
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Expression System
- E.coli
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特异性
- Nucleocapsid from SARS-CoV-2 and SARS-CoV-1
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纯化方法
- Produced in: E.coli
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Labeling Ratio
- a single fluorophore is coupled to exactly one sdAb
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应用备注
- IF: 1:500 (Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g., paraffin-embedded tissue.)
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说明
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Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.
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缓冲液
- lyophilized from PBS pH7.4 with 2% BSA (US-Origin)
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
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别名
- Nucleocapsid from SARS-CoV-2
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背景
- The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.
抗原
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