EAF1 抗体 (AA 50-120)
This anti-EAF1 antibody is a 兔 多克隆 antibody detecting EAF1 in WB. Suitable for 人.
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Quick Overview for EAF1 抗体 (AA 50-120) (ABIN7266919)
抗原
See all EAF1 抗体
EAF1
(ELL Associated Factor 1 (EAF1))
适用
人
宿主
兔
克隆类型
多克隆
标记
This EAF1 antibody is un-conjugated
应用范围
Western Blotting (WB)
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抗原表位
- AA 50-120
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原理
- EAF1 Rabbit pAb
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序列
- LQVGKGDEVT ITLPHIPGST PPMTVFKGNK RPYQKDCVLI INHDTGEYVL EKLSSSIQVK KTRAEGSSKI Q
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交叉反应
- 人, 大鼠
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产品特性
- Polyclonal Antibodies
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纯化方法
- Affinity purification
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免疫原
- Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human EAF1 (NP_149074.3).
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亚型
- IgG
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anti-ELL Associated Factor 1 (EAF1) (AA 1-268) antibody
EAF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-ELL Associated Factor 1 (EAF1) (AA 1-268) antibodyEAF1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- WB,1:500 - 1:2000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- EAF1 (ELL Associated Factor 1 (EAF1))
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别名
- EAF1
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背景
- Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF1,Epigenetics & Nuclear Signaling,EAF1
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分子量
- 17kDa/29kDa
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基因ID
- 85403
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UniProt
- Q96JC9
抗原
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