DDDDK Tag 抗体 (Magnetic Agarose Beads)
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Quick Overview for DDDDK Tag 抗体 (Magnetic Agarose Beads) (ABIN7533455)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb
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序列
- DYKDDDDK
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产品特性
- Tag Antibodies
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纯化方法
- Affinity purification
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免疫原
- A synthetic peptide corresponding to DDDDK tag.
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亚型
- IgG
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anti-DDDDK Tag antibody
WB, ICC 宿主: 小鼠 Monoclonal F-tag-01 unconjugated
anti-DDDDK Tag antibodyWB, IP, IF/ICC, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-DDDDK Tag antibodyWB, ELISA, IF 宿主: 小鼠 Monoclonal M2-1 unconjugated Recombinant Antibody
anti-DDDDK Tag antibodyWB, IP, IF, ICC, IHC 宿主: 小鼠 Monoclonal F-tag-01 unconjugated
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应用备注
- IP,1:50 - 1:100
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide, pH 7.3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Avoid freeze / thaw cycles.
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- DDDDK Tag
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别名
- DDDDK
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背景
- FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.,DDDDK,DDDDK tag,DDDDK-tag,DDDDK
抗原
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