STX1A 抗体 (Intracellular)
Quick Overview for STX1A 抗体 (Intracellular) (ABIN7237876)
抗原
See all STX1A 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-265, Intracellular
-
原理
- A Rabbit Polyclonal Antibody to Syntaxin 1
-
特异性
- Intracellular, N-terminus
-
交叉反应
- 小鼠, 大鼠
-
预测反应
- Human,mouse- 264,265 amino acid residues identical
-
产品特性
- Intracellular, N-terminus
-
纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
-
免疫原
-
Immunogen: GST fusion protein,
Immunogen Sequence: GST fusion protein with the sequence MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA ILASPNPDEKT KEELEELMSD IKKTANKVRSK LKSIEQSIEQ EEGLNRSSAD LRIRKTQH S TLSRKFVEVM SEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSDT KKAVKYQSKA RRKK, corresponding to amino acid residues 1-265 of rat syntaxin 1A
-
亚型
- IgG
-
-
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibody
STX1A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11-1A8 unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-255) antibodySTX1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) antibodySTX1A 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibodySTX1A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibodySTX1A 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (pSer14) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (N-Term) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 2-228) antibodySTX1A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (N-Term) antibodySTX1A 适用: 人, 小鼠, 大鼠, 猴, Pig, Hamster, 非洲爪蟾 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:1000
-
说明
-
Cited Application: IP
Negative Control: (ABIN7237335)
Blocking Peptide: (ABIN7237335)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- STX1A (Syntaxin 1A (Brain) (STX1A))
-
别名
- STX1A
-
背景
-
STX1A, Synaptotagmin-associated 35 kDa protein, P35A, P35-1, Neuron-specific antigen HPC-1,Syntaxin 1 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 1, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 1 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as the plasma membrane protein t-SNARE, which together with the vesicular v-SNARE protein VAMP and the membrane-associated SNAP-25 (synaptosome-associated protein 25 kDa), forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.Syntaxin 1 together with SNAP-25 is the target of the neurotoxin botulinum neurotoxin type C (BoNT/C) which following proteolytic degradation of the proteins causes inhibition of neurotransmitter release 3.
Alternative names: Syntaxin 1, STX1A, Synaptotagmin-associated 35 kDa protein, P35A, P35-1, Neuron-specific antigen HPC-1, -
基因ID
- 116470
-
NCBI登录号
- NM_004603
-
UniProt
- P32851
-
途径
- Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
-
