NHE7 抗体 (Extracellular)
Quick Overview for NHE7 抗体 (Extracellular) (ABIN7237818)
抗原
See all NHE7 (SLC9A7) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 158-171, Extracellular
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原理
- A Rabbit Polyclonal antibody to Na+/H+ Exchanger 7 (NHE7) (extracellular)
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特异性
- Extracellular, 2nd loop.
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat - identical, Human - 13 out of 14 amino acid residues identical
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产品特性
- Extracellular, 2nd loop
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EISPGKINNVEQND, corresponding to amino acid residues 158 - 171 of mouse SLC9A7
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亚型
- IgG
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anti-Sodium Hydrogen Exchanger 7 (SLC9A7) (Internal Region) antibody
SLC9A7 适用: 人, 小鼠, 大鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sodium Hydrogen Exchanger 7 (SLC9A7) antibodySLC9A7 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sodium Hydrogen Exchanger 7 (SLC9A7) (C-Term) antibodySLC9A7 适用: 人, 小鼠, 大鼠, 兔, Cow, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Sodium Hydrogen Exchanger 7 (SLC9A7) (AA 29-78) antibodySLC9A7 适用: 人, 小鼠, 大鼠, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Sodium Hydrogen Exchanger 7 (SLC9A7) (N-Term) antibodySLC9A7 适用: 人, 小鼠, 兔, 豚鼠, 犬, 斑马鱼, 山羊 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7236882)
Blocking Peptide: (ABIN7236882)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))
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别名
- SLC9A7
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背景
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Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7,Na+/H+ exchangers (NHEs) also termed solute carrier family 9 (SLC9), are proteins that function as ion transporters. NHEs are present across all kingdoms of life from prokaryotes to eukaryotes and are found to be crucial in preventing intracellular acidification. NHEs are divided into 3 groups SLC9A (NHE1-9), SLC9B (NHA1 and NHA2) and SLC9C according to their cellular location and their function. NHEs regulates cell volume and pH of the cytosol and organelles1.NHE7 is an NHE that is localized predominantly to the trans-Golgi network (TGN) and post-Golgi vesicles. NHE7 differ from other NHEs by its ability to transport both Na+ and K+ via a H+ gradient-dependent manner2. NHE7 mRNA is expressed in wide range of cells but is especially abundant in the brain, skeletal muscle and various secretory tissues3. The predicted membrane topology of NHE7 consist of 12 transmembrane domains (TMDs) and a single N-glycosylation site at position 145. Similarly to the other SLC9A isoforms NHE7 can be assembled as a homodimer. It was recently found that a missense variant in NHE7 associates with multigenerational nonsyndromic intellectual disability (ID)3.
Alternative names: Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7 -
基因ID
- 84679
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NCBI登录号
- NM_032591
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UniProt
- Q96T83
抗原
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