PTAFR 抗体 (Extracellular)
Quick Overview for PTAFR 抗体 (Extracellular) (ABIN7237758)
抗原
See all PTAFR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 259-274, Extracellular
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原理
- A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)
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特异性
- Extracellular, 3rd loop.
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat - identical,Human - 13 out of 16 amino acid residues identical
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产品特性
- Extracellular, 3rd loop
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR
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亚型
- IgG
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anti-Platelet-Activating Factor Receptor (PTAFR) (AA 231-342) antibody
PTAFR 适用: 人, 小鼠, 大鼠 ELISA, WB, FACS, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Activating Factor Receptor (PTAFR) (AA 297-342) antibodyPTAFR 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Activating Factor Receptor (PTAFR) (AA 1-342) antibodyPTAFR 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet-Activating Factor Receptor (PTAFR) (AA 286-312), (C-Term) antibodyPTAFR 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB26788 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:500
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说明
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Negative Control: (ABIN7236256)
Blocking Peptide: (ABIN7236256)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- PTAFR (Platelet-Activating Factor Receptor (PTAFR))
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别名
- PTAFR
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背景
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PTAFR, PAFR,Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1. PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2. Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.
Alternative names: PTAFR, PAFR -
基因ID
- 19204
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NCBI登录号
- NM_000952
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UniProt
- Q62035
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途径
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin
抗原
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