GPR97 抗体 (Extracellular)
Quick Overview for GPR97 抗体 (Extracellular) (ABIN7237675)
抗原
See all GPR97 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 62-75, Extracellular
-
原理
- A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)
-
特异性
- Extracellular, N-term
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- Mouse,rat - 12 out of 14 amino acid residues identical
-
产品特性
- Extracellular, N-term
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: Peptide CNVENLQRYWLNYE, corresponding to amino acid residues 62 - 75 of human ADGRG3
-
亚型
- IgG
-
-
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
-
说明
-
Negative Control: (ABIN7235457)
Blocking Peptide: (ABIN7235457)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- GPR97 (G Protein-Coupled Receptor 97 (GPR97))
-
别名
- ADGRG3
-
背景
- Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97,Adhesion G protein-coupled receptors (aGPCRs) are one of the five main families in the GPCR superfamily. They act as a molecular switch that regulate many physiological processes, including brain development, ion-water homeostasis, inflammation and cell-fate determination1.One member of the aGPCRs family is the orphan receptor GPR97 (also known as ADGRG3). GPR97 consist of seven-transmembrane domain and a GPCR-Autoproteolysis INducing (GAIN) domain. Auto-cleavage via the GAIN domain produces two subunits: the α-subunit (N-terminal fragment (NTF)) and the β-subunit (C-terminal fragment) that remain non-covalently associated at the cell surface2. GPR97 is mainly expressed in immune cells such as neutrophils, eosinophils, and mast cells. Activation of GPR97 results in reduction in cAMP levels in combination with an increase in downstream effectors of Gβγ, such as SRE and NF-κB3. GPR97 was found to regulate B-cell development in mice and antimicrobial activity in human granulocytes3. In addition, studies have shown that GPR97 is involved in macrophage inflammation in high fat diet-induced obesity in mice4.Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97
-
基因ID
- 222487
-
NCBI登录号
- NM_170776
-
UniProt
- Q86Y34
抗原
-